![]() Taken together, the results indicate that the asepsis of PWN did not cause the loss of pathogenicity.Ĭitation: Zhu L-h, Ye J, Negi S, Xu X-l, Wang Z-l, Ji J-y (2012) Pathogenicity of Aseptic Bursaphelenchus xylophilus. Nematodes were recovered from wilted microcuttings and seedlings inoculated with aseptic PWNs and non-aseptic PWNs, and the asepsis of nematodes recovered from aseptic PWN-inoculated microcuttings and seedlings was reconfirmed by culturing them in NB liquid medium at 30☌ for more than 7 days. The results showed that the aseptic microcuttings and seedlings inoculated with aseptic PWNs or non-aseptic PWNs wilted, while those inoculated with bacterial isolates did not wilt. ![]() massoniana seedlings were also inoculated under aseptic conditions with aseptic PWNs and non-aseptic PWNs. densiflora microcuttings and 7-month-old P. produced in vitro were inoculated under aseptic conditions with aseptic PWNs, non-aseptic PWNs and bacteria isolated from the nematodes. To investigate the pathogenicity of the PWN and its associated bacteria, 3-month-old microcuttings derived from certain clones of Pinus densiflora Siebold & Zucc. Studies have indicated that aseptic PWNs do not cause PWD in aseptic pine trees, while PWNs associated with bacteria cause wilting symptoms. ![]() Although the PWN was thought to be the only pathogenic agent associated with this disease, a potential role for bacterial symbionts in the disease process was recently proposed. However, the pathogenic mechanism of pine wilt disease (PWD) remains unclear. Pine wilt is a disease of pine ( Pinus spp.) caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus.
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